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ANALYSIS BASED ON THE NESTED-PCR AND RT-PCR TECHNIQUES (Polymerase Chain Reaction)
Analyse using nested-PCR for the detection of DNA viruses (WSSV, IHHNV, BP, MBV, and HPV). |
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Short description :
DNA viruses detection (WSSV, IHHNV, BP, MBV y HPV) using nested-PCR technique is based in a double set of cycles amplified (carried out) by a Taq DNA polymerase, using a pair of specific primers from a genomic region of the virus for its detection in shrimp hemocytes, larvae and tissues. Amplified products are analyzed in electrophoresis gel of agarose a 2% stained with ethidium bromide, through ultra-violet light observation (UV).
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Analysis using RT-nested PCR for the detection of RNA viruses (IMNV, TSV, YHV, GAV, LSNV).
Short description :
The detection of IMNV, TSV, YHV, GAV and LSNV viruses using he RT-PCR method is based on an inverse transcriptase that converts viral RNA into Complementary DNA, which is used as a target for the amplification reaction “carried out” by the DNA Taq polymerase, using two pairs of specific primers from a genomic region of the virus, for its detection in shrimp hemocytes, larvae and tissues. Amplified products are analyzed in an electrophoresis gel of agarose at 2% stained with ethidium bromide through its observation with ultra-violet light (UV).
Análisis por RT-nested-PCR para la detección de los virus de ARN (IMNV, TSV, YHV, GAV, LSNV).
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Short description :
La detección de los virus IMNV, TSV, YHV, GAV y LSNV mediante el método de RT-PCR (PCR con transcripción inversa) se basa en la acción de una Transcriptasa inversa que convierte el ARN viral en ADN complementario, el cual sirve de blanco para la reacción de amplificación mediada por la Taq ADN polimerasa, utilizando cebadores específicos de una región genómica de los virus, para su detección en hemocitos, larvas y tejidos del camarón. Los productos amplificados son analizados en un gel de electroforesis de agarosa al 2% teñido con bromuro de etidio, mediante la observación con luz ultravioleta (UV). |
ANALYSIS BASED ON THE LAMP TECHNIQUE (Loop mediated isothermal amplification)
Analysis using LAMP for the detection of the intra-cellular bacteria causing NHP
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Short description :
The detection of intra-cellular bacteria causing NHP (Necrotizing Hepatopacreatitis) using the LAMP method is based on the amplification mediated by Bst DNA polymerase, using specific primers in the genomic region of the rickettsia for its detection in shrimp faeces, larvae and hepathopancreas. Amplified products are analysed in an electrophoresis gel of agarose at 2% stained with ethidium bromide, through the observation of ultra-violet light (UV). |
Analysis by RT-LAMP for the detection of RNA viruses (IMNV, TSV). |
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Short description :
The detection of IMNV or TSV using the RT-LAMP method is based on the action of a reverse transcriptase that converts viral RNA into complementary DNA, which serves as a target for the amplified reaction mediated by Bst DNA polymerase, using specific primers of a genomic region of he IMNV virus, for its detection in shrimp hemocytes, larvae and tissues. Amplified products are analyzed in an electrophoresis gel of agarose at 2% stained with ethidium bromide, through ultra-violet light observation (UV).
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For the implementation of molecular biology laboratories in the agriculture, as well as, the aquaculture sector, Concepto Azul offers complete advising services from equipment investments, personal training to the follow up of investigations for an optimal development of its clients and partners’ activities.
Concepto azul has collaborated with the following public and private laboratories for the scientific and technical creation and advisory. |
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