NUCLEIC ACID EXTRACTION KITS (DNA)		  
  DNA extraction kits for hemocytes, tissues and larvae of Litopenaeus vannamei for PCR and LAMP diagnostics.  
   
Short description:
DNA extraction from hemocytes, tissues and larvae for PCR and LAMP diagnostics consists of the homogenization of cells or tissues in a lysis buffer, followed up by an alcohol precipitation of the nucleic acid, which is later conserved in a buffer that guarantees its stability and integrity until its utilization for the diagnostic test.
  
  DNA Extraction kit from Litopenaeus vannameifaeces for PCR and LAMP diagnostics.  
   
Short description:
DNA extraction from shrimp faeces for PCR and LAMP diagnostics consists first of a washing of the faeces in a sterile saline solution before cells’ lysis and intra-cell bacteria in a buffer lysis, followed by an alcohol precipitation of nucleic acid, which is eventually conserved in a buffer which guarantees its stability and integrity, until its use for diagnostic test.
 
  RNA Extraction kit from hemocytes and larvae of Litopenaeus vannamei for RT-PCR.  
   
Short description:
.RNA extraction from hemocytes and shrimp larva for PCR or LAMP diagnostics consists first of cells or tissues homogenization in a lysis buffer composed of phenol and guanidine isothiocyanate that guarantees integral conservation of RNA. Second, cell contaminants are separated during an organic phase; RNAs are maintained in an aqueous phase. After the aqueous phase transfer, RNAs are precipitated in alcohol and re-suspended in Rnase-free H2O which guarantees its stability and integrity until its use for diagnostic test.
This test is adequate for small quantities of cells and tissues which guarantee a good yield in extracted quantities hence guarantying high sensitivity in the following diagnostic tests.
  
   

 



  
 

NESTED-PCR AND RT-PCR TECHNOLOGY BASED KITS
(Polymerase Chain Reaction)

 

  
 

Nested-PCR based kits for DNA virus detection (WSSV, IHHNV, BP, MBV, HPV)

Short description:
Short Description: DNA viruses’ detection (WSSV, IHHNV, BP, MBV and HPV) using the nested-PCR technique is based on a double set of amplification cycles carried out by the DNA Taq polymerase, using a pair of specific primers from a genomic region of the virus for its detection in hemocytes, larvae and tissues of shrimps. Amplified products are analysed in an electrophoresis gel of agarose at 2% stained with ethidium bromide through its observation with ultraviolet light (UV).
 
       
 

Nested-PCR Kit for intracellular bacteria detection causing NHP.

  
   

Short description::
The detection of the intracellular bacteria causing NHP (Necrotizing Hepatopancreatitis) using the nested-PCR technique is based on a double set of amplification cycles “carried out” by DNA Taq polymerase, using a pair of specific primers of a genomic region of the 16S DNA, for its detection in shrimp hemocyte, larvae and tissues.
Amplified products are analysed in an electrophoresis gel of agarose at 2% stained with ethidium bromide, through ultra-violet light.

  
       
       
 
RT-nested PCR kit for the detection of RNA viruses (IMNV, TSV, YHV, GAV, LSNV).

Short description:

The detection of  IMNV, TSV, YHV, GAV and LSNV viruses using he RT-PCR method is based on an reverse transcriptase that converts viral RNA into complementary DNA, which is used as a target for the amplification reaction carried out by the DNA Taq polymerase, using two pairs of specific primers from a genomic region of the virus, for its detection in shrimp hemocytes, larvae and tissues. Amplified products are analyzed in an electrophoresis gel of agarose at 2% stained with ethidium bromide through its observation with ultra-violet light (UV).
 
       
       
       
       
       
 
 
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